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Name: Leung Kin Man University number: 2009109720 Date of try: 25-2-2010 BIOL1133: biologic Science Laboratory Course Experiment 4: Expression of homos secretin sense organ concretion protein in E coli. Aim: The suggest of the experiment is to express human secretin receptor fusion protein in E coli by conducting SDS-PAGE gel ionophoresis and estimate the molecular size of that protein. Abstract: In this experiment, the human secretin receptor fusion protein was expressed exploitation the SDS-PAGE technique. The pre-induced and the sample after(prenominal) IPTG induction was use to extract the human secretin receptor protein by utilise denaturing buffer zone and elution buffer. The molecular weight of the protein croup also be estimated by comparing the bands on the protein ladder. gateway: Massive production of active protein is usually done by recombinant deoxyribonucleic acid technology. E coli. was employ as vector for launching and expression of fusion protein. E coli. is a germ-negative bacteria with forked membrane layer and gamin plasmid. In this experiment, the N-terminal extracellular nation of the human secretin receptor in E coli. was expressed using IPTG inducible system. Secretin is the key hormone that prudent for the regulation of gastrointestinal wait on by regulating the secretion of bicarbonate and enzyme from the exocrine pancreas. This kindle help to neutralize stomachic acid (hydrochloric acid) and hence prevail an optimum pH care for for intestinal digestion. The action of secretin is mainly controlled by high-affinity membrane receptors distributed in our body and it is tissue-specific. The N-terminal cryptograph locality of the extracellular domain of the receptor protein is obtained by conducting polymerase chain reaction (PCR) mutagenesis method. This technique was achieved by using specific deoxyribonucleic acid primers and DNA strand as a template to start the PCR reaction. The cod ing sequence was introduced into the bacteri! al DNA as a pallbearer of the coding sequence. Upon IPTG...If you want to posture a full essay, cabaret it on our website: BestEssayCheap.com

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